Date of Award

2023

Document Type

Master Thesis

Degree Name

Masters of Science (Research)

Department

BIOLOGICAL SCIENCES

First Advisor

Dr. Linda Giblin

Second Advisor

Dr Michael Callanan

Abstract

Increasing the feeling of fullness through modulation of satiety hormones by dietary proteins may be a promising daily approach in the prevention of unnecessary weight gain and in the maintenance of a healthy weight over our lifetime. The effects of animal sourced proteins on satiety hormone (GLP-1, PYY and CCK) has been well documented. However to meet the EU Green Deal of net zero greenhouse gas emissions by 2050, a 15-20 % dietary shift to plant proteins is required. This thesis examined the satiating potential of six plant protein ingredients, Teff, Oat Fibre, Lentil Protein Isolate, Buckwheat, Quinoa and Strong Wheat Flour as they transited the upper gut by quantifying satiety hormone response (GLP-1, PYY, CCK) in enteroendocrine cells, in vitro. To look at food matrix, the satiating potential of three plant protein products were also examined in the form of protein milks, Lentil milk, Soy milk and Oat milk. The effects of these gastrointestinal-digested samples on the health of the gut barrier was also investigated using the well-established barrier model, Caco2-HT29-MTX co-culture. A 10kDa fraction of gastrointestinal digested Buckwheat was capable of significantly increasing active GLP-1 secretion while digested buckwheat maintained gut barrier integrity and health.

Access Level

info:eu-repo/semantics/openAccess

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