Abstract
CHAPK is the N-terminal cysteine, histidine-dependent amidohydrolase/peptidase domain (CHAP domain) of the Staphylococcus aureus bacteriophage K endolysin LysK. It is formed from the first 165 residues of LysK and functions by cleaving specific peptidoglycan peptide bonds, causing bacterial lysis. CHAPK can lyse S. aureus when applied exogenously, making it a good candidate for the treatment of multidrug-resistant Staphylococcus aureus infections. Here, the crystallization of CHAPK and the collection of native and derivative data to high resolution, which allowed structure solution, are reported. The structure may help to elucidate the mechanism of action and in the design of chimeric proteins or mutants with improved antibacterial activity.
Disciplines
Biology
DOI
10.1107/S1744309113030133
Full Publication Date
November 2013
Publisher
Wiley
Resource Type
journal article
Access Rights
metadata only access
Alternative Identifier
https://scripts.iucr.org/cgi-bin/paper?S1744309113030133
Recommended Citation
Sanz-Gaitero, M. et al., 2013. Crystallization of the CHAP domain of the endolysin fromStaphylococcus aureusbacteriophage K. Acta Crystallographica Section F Structural Biology and Crystallization Communications, 69(12), pp.1393–1396. Available at: http://dx.doi.org/10.1107/S1744309113030133.
Publication Details
Acta Crystallographica Section F Structural Biology and Crystallization Communications.