The truncated phage lysin CHAP_k eliminates staphylococcus aureus in the nares of mice

Authors

Mark Fenton, Cork Institute of Technology
Pat G. Casey, APC Microbiome Ireland
Colin Hill, APC Microbiome Ireland
Cormac G.M. Gahan, APC Microbiome Ireland
R. Paul Ross, Moorepark Food Research Centre
Olivia Mcauliffe, Moorepark Food Research Centre
Jim O'Mahony, Cork Institute of Technology
Fiona Maher, Cork Institute of Technology
Aidan Coffey, Cork Institute of Technology

Abstract

The endolysin LysK derived from staphylococcal phage K has previously been shown to have two enzymatic domains, one of which is an N-acetylmuramoyl-L-alanine amidase and the other a cysteine/histidine dependant amidohydrolase/ peptidase designated CHAPk. The latter, when cloned as a single-domain truncated enzyme, is conveniently overexpressed in a highly-soluble form. This enzyme was shown to be highly active in vitro against live cell suspensions of S. aureus. In the current study, the IVIS imaging system was used to demonstrate the effective elimination of a lux labeled S. aureus from the nares of BALB/c mice. © 2010 Landes Bioscience.

Disciplines

Biology

DOI

10.4161/bbug.1.6.13422

Full Publication Date

January 2010

Publication Details

Bioengineered Bugs

Publisher

Taylor & Francis

Funder Name 1

Science Foundation Ireland

Award Number 1

Ref 05/ RF/BIM004

Resource Type

journal article

Access Rights

open access

License Condition

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

Fenton, Mark; Casey, Pat G.; Hill, Colin; Gahan, Cormac G.M.; Ross, R. Paul; Mcauliffe, Olivia; O'Mahony, Jim; Maher, Fiona; and Coffey, Aidan, "The truncated phage lysin CHAP_k eliminates staphylococcus aureus in the nares of mice" (2010). Department of Biological Sciences Publications [online].
Available at: https://doi.org/10.4161/bbug.1.6.13422