Insertional inactivation of determinants for Mg2+ and Co 2+ transport as a tool for screening recombinant Lactococcus species clones
Abstract
Insertional inactivation of the plasmid-encoded determinants for Mg 2+ and Co2+ transport, orf18/corA, provides a tool for screening recombinant clones in Lactococcus, based on the observation that overexpression of orfl8/corA results in cell growth inhibition on certain concentrations of CoCl2. The lacticin 3147 immunity gene, ltnI, was used to insertionally inactivate orfl8/corA. The resulting clones were capable of growth on concentrations of CoCl2 that were inhibitory to the parent strain. Since only 3 of 17 lactococcal starters naturally harbor cor A, the system has potential as a screen for selecting recombinant lactococcal clones. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Disciplines
Biology
DOI
10.1128/AEM.71.8.4897-4901.2005
Full Publication Date
August 2005
Publisher
American Society for Microbiology
Resource Type
journal article
Access Rights
open access
License Condition
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Mills, Susan; Coffey, Aidan; Hill, Colin; Fitzgerald, Gerald F.; McAuliffe, Olivia; and Ross, R. Paul, "Insertional inactivation of determinants for Mg2+ and Co 2+ transport as a tool for screening recombinant Lactococcus species clones" (2005). Department of Biological Sciences Publications [online].
Available at: https://doi.org/10.1128/AEM.71.8.4897-4901.2005
Publication Details
Applied and Environmental Microbiology