An in vitro investigation of the survival and/or growth of Campylobacter jejuni in broiler digestate from different feed types

Author Email for Access Request

aidan.coffey@cit.ie

Abstract

Campylobacter spp. is the leading cause of bacterial gastroenteritis worldwide and poultry are the primary reservoir. The aim of this study was to investigate the survival and/or growth of Campylobacter jejuni NCTC 11168 in broiler digestate prepared from commercial starter, grower and finisher feed formulations. Bolton broth and digestates were prepared, inoculated with C. jejuni NCTC 11168 (approximately 3 log10 CFU per ml) and incubated under microaerobic conditions at 42°C for 24 h. Samples were taken at t = 0 (immediately after inoculation) and every 3 h thereafter, serially diluted and plated onto mCCDA. Campylobacter jejuni grew as expected in Bolton broth (control) reaching the early stationary phase after approximately 15 h. In contrast, although bacterial concentrations were maintained for at least 9 h, none of the feed digestates supported the growth of C. jejuni, which were not detected after 15 h. It is suggested that the nutrients available in the feed digestates are not enough to support C. jejuni growth and that additional factors may be at play in the avian gastrointestinal tract.

Disciplines

Biology

DOI

10.1111/lam.13390

Full Publication Date

January 2021

Publication Details

Letters in Applied Microbiology

© 2020 The Society for Applied Microbiology

Publisher

Wiley

Funder Name 1

Department of Agriculture, Food and the Marine

Award Number 1

5F641

Funder Name 2

Teagasc

Resource Type

journal article

Access Rights

metadata only access

Alternative Identifier

https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/lam.13390

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