Erwinia amylovora phage vB_EamM_Y3 represents another lineage of hairy Myoviridae

ORCID

https://orcid.org/0000-0003-4116-9913

Author Email for Access Request

aidan.coffey@cit.ie

Abstract

To date, a small number of jumbo myoviruses have been reported to possess atypical whisker-like structures along the surface of their contractile tails. Erwinia amylovora phage vB_EamM_Y3 is another example. It possesses a genome of 261,365 kbp with 333 predicted ORFs. Using a combination of BLASTP, Interproscan and HHpred, about 21% of its putative proteins could be assigned functions involved in nucleotide metabolism, DNA replication, virion structure and cell wall degradation. The phage was found to have a signal-arrest-release (SAR) endolysin (Y3_301) possessing a soluble lytic transglycosylase domain. Like other SAR endolysins, inducible expression of Y3_301 caused Escherichia coli lysis, which is dependent on the presence of an N-terminal signal sequence. Phylogenetic analysis showed that its closest relatives are other jumbo phages including Pseudomonas aeruginosa phage PaBG and P. putida phage Lu11, sharing 105 and 87 homologous proteins respectively. Like these phages, Y3 also shares a distant relationship to Ralstonia solanacearum phage ΦRSL1 (sharing 55 homologous proteins). As these phages are unrelated to the Rak2-like group of hairy phages, Y3 along with Lu11 represent a second lineage of hairy myoviruses.

Disciplines

Biology

DOI

10.1016/j.resmic.2018.04.006

Full Publication Date

November 2018

Publication Details

Research in Microbiology

© 2018 Institut Pasteur and © 2018 Elsevier

Publisher

Elsevier

Funder Name 1

Science Foundation Ireland

Award Number 1

12/R1/2335

Resource Type

journal article

Access Rights

open access

Alternative Identifier

https://www.sciencedirect.com/science/article/pii/S0923250818300627?via%3Dihub

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