First Evidence of Production of the Lantibiotic Nisin P

Authors

Enriqueta Garcia-Gutierrez, Gut Microbes and Health, Quadram Institute Bioscience, Norwich, NR4 7UQ, UK; 2 Teagasc Food Research Centre, Moorepark, Cork, P61 C996, Ireland
Paula M. O'Connor, 2 Teagasc Food Research Centre, Moorepark, Cork, P61 C996, Ireland; 3 APC Microbiome Ireland, University College Cork, Cork, T12 K8AF, Ireland
Gerhard Saalbach, 4 Biological Chemistry, John Innes Centre, Norwich, NR4 7UH, UK
Calum J. Walsh, 2 Teagasc Food Research Centre, Moorepark, Cork, P61 C996, Ireland; 3 APC Microbiome Ireland, University College Cork, Cork, T12 K8AF, Ireland
James W. Hegarty, 2 Teagasc Food Research Centre, Moorepark, Cork, P61 C996, Ireland; 3 APC Microbiome Ireland, University College Cork, Cork, T12 K8AF, Ireland
Caitriona M. Guinane, Department of Biological Sciences, Cork Institute of Technology, Cork, Ireland; 2 Teagasc Food Research Centre, Moorepark, Cork, P61 C996, IrelandFollow
Melinda J. Mayer, Gut Microbes and Health, Quadram Institute Bioscience, Norwich, NR4 7UQ, UK
Arjan Narbad, Gut Microbes and Health, Quadram Institute Bioscience, Norwich, NR4 7UQ, UK
Paul D. Cotter, 2 Teagasc Food Research Centre, Moorepark, Cork, P61 C996, Ireland; 3 APC Microbiome Ireland, University College Cork, Cork, T12 K8AF, Ireland

ORCID

https://orcid.org/0000-0001-8215-1387

Abstract

Nisin P is a natural nisin variant, the genetic determinants for which were previously identifed in the genomes of two Streptococcus species, albeit with no confrmed evidence of production. Here we describe Streptococcus agalactiae DPC7040, a human faecal isolate, which exhibits antimicrobial activity against a panel of gut and food isolates by virtue of producing nisin P. Nisin P was purifed, and its predicted structure was confrmed by nanoLC-MS/MS, with both the fully modifed peptide and a variant without rings B and E being identifed. Additionally, we compared its spectrum of inhibition and minimum inhibitory concentration (MIC) with that of nisin A and its antimicrobial efect in a faecal fermentation in comparison with nisin A and H. We found that its antimicrobial activity was less potent than nisin A and H, and we propose a link between this reduced activity and the peptide structure.

Disciplines

Biology | Life Sciences

DOI

10.1038/s41598-020-60623-0

Full Publication Date

February 2020

Publication Details

Scientific Reports

© 2020 Authors

Publisher

Nature Research

Funder Name 1

Teagasc

Award Number 1

2015066

Funder Name 2

Biotechnology and Biological Sciences Research Council

Award Number 2

BB/R012490/1 and BBS/E/F/000PR10356

Funder Name 3

Science Foundation Ireland

Award Number 3

SFI/11/PI/113

Award Number 4

SFI/12/RC/2273

Resource Type

review

Access Rights

open access

License Condition

This work is licensed under a Creative Commons Attribution 4.0 International License.

Alternative Identifier

https://www.nature.com/articles/s41598-020-70229-1

Recommended Citation

Garcia-Gutierrez, E., O’Connor, P.M., Saalbach, G. et al. First evidence of production of the lantibiotic nisin P. Sci Rep 10, 3738 (2020). https://doi.org/10.1038/s41598-020-60623-0