Abstract
Cronobacter sakazakii is a neonatal pathogen responsible for up to 80% of fatalities in infected infants. Low birth weight infants and neonates infected with C. sakazakii suffer necrotizing enterocolitis, bacteraemia and meningitis. The mode of transmission most often associated with infection is powdered infant formula (PIF) which, with an aw of ∼0.2, is too low to allow most microorganisms to persist. Survival of C. sakazakii in environments subject to extreme hyperosmotic stress has previously been attributed to the uptake of compatible solutes including proline and betaine. Herein, we report the construction and screening of a C. sakazakii genome bank and the identification of ProP (ESA_02131) as a carnitine uptake system.
Disciplines
Bacteriology | Biochemistry | Biology | Biotechnology | Food Microbiology | Genetics and Genomics | Genomics | Microbial Physiology | Microbiology | Organismal Biological Physiology | Pathogenic Microbiology
DOI
10.1080/21655979.2015.1043500
Full Publication Date
April 2015
Publisher
Taylor & Francis
Funder Name 1
European Commission
Award Number 1
324365
Funder Name 2
Irish Research Council of Science Engineering and Technology (IRCSET)
Award Number 2
RS/2010/2300
Resource Type
journal article
Access Rights
restricted access
Open Access Route
Bronze Open Access
Alternative Identifier
https://www.tandfonline.com/doi/full/10.1080/21655979.2015.1043501
Recommended Citation
Feeney, A. & Sleator R.D. (2015) Functional Screening of the Cronobacter sakazakii BAA-894 Genome reveals a role for ProP (ESA_02131) in carnitine uptake, Bioengineered, 6:3, 161-165, DOI: 10.1080/21655979.2015.1043500
Bacterial strains and plasmids
Table 2. Primers.csv (1 kB)
Primers
Included in
Bacteriology Commons, Biochemistry Commons, Biology Commons, Biotechnology Commons, Food Microbiology Commons, Genomics Commons, Microbial Physiology Commons, Organismal Biological Physiology Commons, Pathogenic Microbiology Commons
Publication Details
Bioengineered