Meta-Hort- How agronomic factors affect the microbiome of the Horticulture Produce

Presenter Information

Maryada BohraFollow

ORCID

https://orcid.org/0009-0001-4598-6622

Department

Biological Sciences

Year of Study

3

Full-time or Part-time Study

Full-time

Level

Postgraduate

Name of Course

Doctor of Philosophy

Presentation Type

Oral Presentation

Supervisor

Dr Micheal Gaffney

Supervisor

Dr Caitriona Guinane

Supervisor

Dr Orla O’Sullivan

Abstract

Fresh fruits and vegetables are increasingly recognised as reservoirs of complex microbial communities with potential implications for host health. While their nutritional value is well established, the influence of agronomic variables, such as cultivation substrate, harvest timing, and postharvest handling, on the structure and function of produce-associated microbiota remains insufficiently characterised.

This study investigates how pre- and postharvest factors modulate the surface microbiome of fruits and vegetables, using an integrated approach that combines culture-based microbiology, shot-gun sequencing, and ex-vivo gut models. Microbial community composition and functional capacity are being assessed, with short-chain fatty acid (SCFA) production serving as a key indicator of potential gut health benefits.

Preliminary data suggest that production variables significantly influence microbial diversity and functional output, with specific taxa enriched under defined conditions. Select isolates exhibiting probiotic potential have also been identified from strawberry and mushroom surfaces. These insights advance our understanding of how horticultural practices intersect with microbial ecology and inform strategies to enhance the health-promoting potential of fresh produce.

Keywords:

produce microbiome, gut health, postharvest storage, strawberries, broccoli, probiotics

Start Date

16-6-2025 1:45 PM

End Date

16-6-2025 2:00 PM

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Jun 16th, 1:45 PM Jun 16th, 2:00 PM

Meta-Hort- How agronomic factors affect the microbiome of the Horticulture Produce

Fresh fruits and vegetables are increasingly recognised as reservoirs of complex microbial communities with potential implications for host health. While their nutritional value is well established, the influence of agronomic variables, such as cultivation substrate, harvest timing, and postharvest handling, on the structure and function of produce-associated microbiota remains insufficiently characterised.

This study investigates how pre- and postharvest factors modulate the surface microbiome of fruits and vegetables, using an integrated approach that combines culture-based microbiology, shot-gun sequencing, and ex-vivo gut models. Microbial community composition and functional capacity are being assessed, with short-chain fatty acid (SCFA) production serving as a key indicator of potential gut health benefits.

Preliminary data suggest that production variables significantly influence microbial diversity and functional output, with specific taxa enriched under defined conditions. Select isolates exhibiting probiotic potential have also been identified from strawberry and mushroom surfaces. These insights advance our understanding of how horticultural practices intersect with microbial ecology and inform strategies to enhance the health-promoting potential of fresh produce.