Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue

Authors

James Carroll, Cork Institute of Technology
Pierre Douarre, Cork Institute of Technology
Aidan Coffey, Department of Biological Sciences, Cork Institute of Technology, Cork, IrelandFollow
Jim Buckley, Cork County Council
Bill Cashman, Cork County Council
Kevin O'Farrell, Cork County Council
Jim O'Mahony, Department of Biological Sciences, Cork Institute of Technology, Cork, IrelandFollow

Abstract

A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 104 to 108 CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this method has applications for high-throughput screening against M. avium subsp. paratuberculosis. Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Disciplines

Biology

DOI

10.1128/AEM.01203-09

Full Publication Date

December 2009

Publication Details

Applied and Environmental Microbiology

Publisher

American Society for Microbiology

Resource Type

journal article

Access Rights

open access

License Condition

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

Carroll, James; Douarre, Pierre; Coffey, Aidan; Buckley, Jim; Cashman, Bill; O'Farrell, Kevin; and O'Mahony, Jim, "Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue" (2009). Department of Biological Sciences Publications [online].
Available at: https://doi.org/10.1128/AEM.01203-09