Inhibition of Listeria monocytogenes biofilms by bacteriocin-producing bacteria isolated from mushroom substrate

Authors

A. S. Bolocan, Moorepark Food Research Centre
V. Pennone, Moorepark Food Research Centre
P. M. O'Connor, Moorepark Food Research Centre
A. Coffey, Department of Biological Sciences, Cork Institute of Technology, Cork, IrelandFollow
A. I. Nicolau, Universitatea Dunarea de Jos din Galati
O. McAuliffe, Moorepark Food Research Centre
K. Jordan, Moorepark Food Research Centre

ORCID

https://orcid.org/0000-0003-4116-9913

Author Email for Access Request

aidan.coffey@cit.ie

Abstract

Aims: This study was designed to investigate the ability of naturally occurring bacteria isolated from mushroom substrate to prevent biofilm formation by Listeria monocytogenes or to remove existing biofilms in mushroom production facilities. Methods and Results: It is generally recognized that L. monocytogenes forms biofilms that can facilitate its survival in food-processing environments. Eleven bacteriocin-producing isolates were identified and the bacteriocins characterized based on heat and enzyme inactivation studies. Further characterization was undertaken by MALDI-TOF mass spectrometry, PCR and sequencing. Production of nisin Z (by Lactococcus lactis isolates), subtilomycin (by Bacillus subtilis isolates) and lichenicidin (by Bacillus licheniformis and Bacillus sonorensis isolates) was detected. In co-culture with L. monocytogenes, the bacteriocin-producing strains could prevent biofilm formation and reduce pre-formed biofilms. Conclusions: Mushroom substrate can be a source of bacteriocin-producing bacteria that can antagonize L. monocytogenes. Significance and Impact of the Study: The results highlight the potential of bacteriocin-producing strains from mushroom substrate to reduce L. monocytogenes biofilm in food production environments, contributing to a reduction in the risk of food contamination from the environment.

Disciplines

Biology

DOI

10.1111/jam.13337

Full Publication Date

January 2017

Publication Details

Journal of Applied Microbiology

© 2016 The Society for Applied Microbiology

Publisher

Wiley

Funder Name 1

Irish Department of Agriculture, Food and Marine

Award Number 1

14/F/881

Funder Name 2

COST Action

Award Number 2

FA1202

Resource Type

journal article

Access Rights

open access

Alternative Identifier

https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/jam.13337

Recommended Citation

Bolocan, A.S. et al., 2016. Inhibition of Listeria monocytogenesbiofilms by bacteriocin-producing bacteria isolated from mushroom substrate. Journal of Applied Microbiology, 122(1), pp.279–293. Available at: http://dx.doi.org/10.1111/jam.13337.