A rapid and sensitive system for recovery of nucleic acids from Mycobacteria sp. on archived glass slides

Authors

Balkis A. Talip, School of Biomedical Sciences, Faculty of Life and Health Sciences, Ulster University, Cromore Road, BT52 1SA, Coleraine, County Londonderry, Northern Ireland & Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia, 84000 Pagoh, Muar, Johor, Malaysia
William J. Snelling, School of Biomedical Sciences, Faculty of Life and Health Sciences, Ulster University, Cromore Road, BT52 1SA, Coleraine, County Londonderry, Northern Ireland
Roy D. Sleator, Department of Biological Sciences, Cork Institute of Technology
Colm Lowery, School of Biomedical Sciences, Faculty of Life and Health Sciences, Ulster University, Cromore Road, BT52 1SA, Coleraine, County Londonderry, Northern Ireland
James S.G. Dooley, School of Biomedical Sciences, Faculty of Life and Health Sciences, Ulster University, Cromore Road, BT52 1SA, Coleraine, County Londonderry, Northern Ireland

Abstract

The field of diagnostics continues to advance rapidly with a variety of novel approaches, mainly dependent upon high technology platforms. Nonetheless much diagnosis, particularly in developing countries, still relies upon traditional methods such as microscopy. Biological material, particularly nucleic acids, on archived glass slides is a potential source of useful information both for diagnostic and epidemiological purposes. There are significant challenges faced when examining archived samples in order that an adequate amount of amplifiable DNA can be obtained. Herein, we describe a model system to detect low numbers of bacterial cells isolated from glass slides using (laser capture microscopy) LCM coupled with PCR amplification of a suitable target.

Disciplines

Analytical, Diagnostic and Therapeutic Techniques and Equipment | Biochemistry, Biophysics, and Structural Biology | Microbiology

DOI

10.1186/s12866-018-1335-0

Full Publication Date

November 2018

Publication Details

BMC Microbiology

Publisher

Springer

Funder Name 1

Ministry of Higher Education Malaysia

Funder Name 2

Universiti Tun Hussein Onn Malaysia

Resource Type

journal article

Access Rights

open access

Open Access Route

Gold Open Access

License Condition

This work is licensed under a Creative Commons Attribution 4.0 International License.

Alternative Identifier

https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-018-1335-0

Recommended Citation

A. Talip, B., Snelling, W.J., Sleator, R.D. et al. A rapid and sensitive system for recovery of nucleic acids from Mycobacteria sp. on archived glass slides. BMC Microbiol 18, 196 (2018). https://doi.org/10.1186/s12866-018-1335-0